nxCode - DNA Barcode Designer and Decoderfor Next-Gen Sequencing |
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Main | Download barcode sets | Download software | Usage | Algorithmic details | FAQ |
nxCode
- DNA Barcode Designer and Decoder
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Page contents | |||||
Introduction | |||||
nxCode
is a DNA
barcode-set design and decode tool, optimized for experiments on
next-gen
sequencers. nxCode allows the user to produce sets of barcodes, and
then decode results from experiments incorporating these
barcodes. nxCode and this website allow you to: 1. Download DNA barcode sets optimized for experiments using Illumina's Genome Analyzer(Solexa). 2. Design your own barcode sets (meeting your own custom constraints) using nxCodeBuilder. 3. Decode your data after sequencing, using nxDecoder. |
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What is nxCode for? | |||||
nxCode is meant
to be used when sequencing DNA from multiple sources in a single run
on a next-gen sequencer. Samples from each source can be
assigned unique "barcodes" (short DNA tags, usually up to 10
nucleotides in length, that are attached to each sample by PCR before
sequencing). After sequencing, the barcodes can be used to
determine the origins of each sequence. The barcodes are designed so that each barcode will still be uniquely identifiable even after the introduction of sequencing errors and oligonucleotide synthesis errors. Barcodes currently available on this website are optimized for Illumina's Genome Analyzer, but are also suitable for use on other platforms. |
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Authors | |||||
Oron Navon (oronn@bgu.ac.il) Roy Ronen (royr@bgu.ac.il) Yaniv Erlich (erlich@cshl.edu) Mitchell Bekritsky (bekritsk@cshl.edu) |
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Useful links | |||||
nxCode was developed in the Hannon Lab at Cold Spring Harbor Laboratory. |