FASTX-Toolkit

FASTQ/A short-reads pre-processing tools
Here are screen-shots of the tool's pages in Galaxy.

Galaxy Usage

Galaxy is web-based framework for experimental and computational biologists.
With Galaxy one can easily use analysis tools through a web-based interface.

If you're using the public Galaxy web-site, FASTX-toolkit tools are already available for general use.
If you're running a local mirror of the galaxy server, follow the Installation instructions to integrate the tools into your Galaxy server.

Inside Galaxy, click on FASTA/Q Information or FASTA/Q Manipulation categories to access the FASTX-Toolkit tools:


Each tool contains a optional parameters and a short description. Click on the different tools to see their help screen.

FASTA/Q Information tools (screen-shots from Galaxy)

Quality Statistics


Quality Boxplot



Nucleotide Distribution


FASTA/Q Manipulation Tools (screen-shots from Galaxy)

FASTA/Q Clipper



FASTA/Q Trimmer


FASTA/Q End Trimmer


FASTQ Quality Trimmer


FASTA/Q Renamer


FASTA Collapser


FASTA UnCollapser


UnCollapse row (in a text file)


Artifacts Filter


FASTQ Quality Filter


FASTQ/A Reverse Complement


FASTQ-to-FASTA converter


FASTA Formatter


FASTA Nucleotide Changer


FASTA Clipping Histogram


FASTQ/A barcode splitter